Effect of Sodium Hypochlorite 0.05% in Chronic Wounds – Journal of Clinical Medicine

We’re thrilled to present the paper published by the Journal of Clinical Medicine, in which one of our dear colleagues -Giorgina Mangano, is a collaborating author.

In particular, this paper concentrates on the effects of Sodium Hypochlorite 0.05% in Chronic Wounds. In such circumstances, it has been shown that high concentrations of matrix metalloproteinases (MMPs) can cause excessive proteolysis and slow wound healing. Thus, restoring a proper MMP balance can help reduce the risk of a chronic wound.

In this study, the in vitro activity of Sodium Hypochlorite solution was assessed on the release of MMPs, using differentiated THP-1 macrophage cell line. Moreover, the THP-1 cells were stimulated with wound fluid from patients with active venous leg ulcers in the inflammatory phase. The effect of Sodium Hypochlorite was also evaluated on healthy human peripheral blood serum samples.

The findings indicate a potential capacity of the Sodium Hypochlorite solution to influence the proteolytic processes within the wound microenvironment, providing additional insights into its function and clinical application in wound care. In fact, Sodium Hypochlorite downregulated the MMP-9 activity and its activated form, not only in the macrophages differentiated from the THP-1 cell line, but also in peripheral blood serum. Particularly, a reduction of MMP-1 and MMP-12 release was also observed.

To gain further insight in this research project, check the full paper, which is freely available online! Click here for a quick redirection.


Maniscalco R, Mangano G, de Joannon AC, Vergassola M, Zucchi S, Mannello F, Ragni L. Effect of Sodium Hypochlorite 0.05% on MMP-9 Extracellular Release in Chronic Wounds. J Clin Med. 2023 Apr 28;12(9):3189. doi: 10.3390/jcm12093189. PMID: 37176629; PMCID: PMC10179022.